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Biology
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通过集成活细胞监测,自动生产人类诱导多能干细胞衍生皮质神经元和多巴胺神经元
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
Please note that all translations are automatically generated.
Click here for the English version.
通过集成活细胞监测,自动生产人类诱导多能干细胞衍生皮质神经元和多巴胺神经元
DOI:
10.3791/61525-v
•
09:34 min
•
August 06, 2020
•
Ashutosh Dhingra*
1
,
Joachim Täger*
1
,
Elisangela Bressan*
1
,
Salvador Rodriguez-Nieto
,
Manmeet-Sakshi Bedi
,
Stefanie Bröer
,
Eldem Sadikoglou
,
Noémia Fernandes
,
Melissa Castillo-Lizardo
,
Patrizia Rizzu
,
Peter Heutink
3
1
Genome Biology of Neurodegenerative Diseases
,
German Center for Neurodegenerative Diseases (DZNE)
,
2
Applied Genomics for Neurodegenerative Diseases
,
German Center for Neurodegenerative Diseases (DZNE)
,
3
Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research
,
University of Tübingen
Chapters
00:05
Introduction
00:43
New Culture Plate and Tip Loading and Decontamination
01:35
Culture Medium and/or Dissociation Reagent Filling
02:09
Automated Method Execution
02:51
Human Induced Pluripotent Stem Cell (hiPSC) Seeding
04:09
Automated Confluence Assessment
04:47
Medium Change
05:37
Subcultivation
06:42
Automated High Content, High Throughput Imaging
07:14
Results: Representative Automated System-Produced hiPSC-Derived Cortical and Dopaminergic Neuron Characterization
08:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们展示人类诱导多能干细胞 (hiPSC) 培养物的自动化和与自动成像和分析相容的神经元分化。
Tags
Automated Production
Human Induced Pluripotent Stem Cells
Cortical Neurons
Dopaminergic Neurons
Live-cell Monitoring
Standardized Procedures
Low Experimental Variation
High Phenotypic Reproducibility
Graphic User Interface
Resource Instrument Process
HEPA Hood
Decontamination
UV Radiation
Liquid Handling Station
Culture Medium
Dissociation Reagent
Cell Line
Project
Batch
Seeding
Brightfield Imaging Cytometer
Cell Counting
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