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Afbødning af blodbårne celle vedhæftet fil til metal implantater gennem CD47-Afledt peptid immobilisering
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
DOI:

08:13 min

December 03, 2020

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Chapters

  • 00:04Introduction
  • 00:50Coating Bare Metal Surfaces with PEI-PDT
  • 02:37Attaching Human PepCD47 to PEI-PDT Modified Surfaces
  • 03:21Chandler Loop for Analyzing Cellular Attachment to Metal Surfaces
  • 04:54Analyzing Cellular Attachment to Metal Surfaces using CFDA Dye
  • 05:48Results: Anti-inflammatory Properties of PepCD47 Coated Metal Surfaces Exposed to Whole Blood and Isolated Monocytes
  • 07:16Conclusion

Summary

Automatic Translation

Præsenteret her er en protokol for tilføjelse peptid CD47 (pepCD47) til metal stents ved hjælp af polybisfosfonat kemi. Funktionalisering af metal stents ved hjælp af pepCD47 forhindrer fastgørelse og aktivering af inflammatoriske celler og dermed forbedre deres biokompatibilitet.

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