Journal
/
/
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
DOI:

08:13 min

December 03, 2020

, , , , ,

Chapters

  • 00:04Introduction
  • 00:50Coating Bare Metal Surfaces with PEI-PDT
  • 02:37Attaching Human PepCD47 to PEI-PDT Modified Surfaces
  • 03:21Chandler Loop for Analyzing Cellular Attachment to Metal Surfaces
  • 04:54Analyzing Cellular Attachment to Metal Surfaces using CFDA Dye
  • 05:48Results: Anti-inflammatory Properties of PepCD47 Coated Metal Surfaces Exposed to Whole Blood and Isolated Monocytes
  • 07:16Conclusion

Summary

Automatic Translation

Hier wird ein Protokoll zum Anhängen von Peptid CD47 (pepCD47) an Metallstents mit Polybisphosphonatchemie vorgestellt. Die Funktionalisierung von Metallstents mit pepCD47 verhindert die Anhaftung und Aktivierung von Entzündungszellen und verbessert so deren Biokompatibilität.

Related Videos

Read Article