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通过CD47衍生肽固定，缓解血系细胞附着到金属植入物
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Bioengineering
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JoVE Journal Bioengineering
Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
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通过CD47衍生肽固定，缓解血系细胞附着到金属植入物

DOI:
10.3791/61545-v

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08:13 min

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December 03, 2020

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, , , , ,
1The Children’s Hospital of Philadelphia, 2Department of Pediatrics, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:04Introduction
  • 00:50Coating Bare Metal Surfaces with PEI-PDT
  • 02:37Attaching Human PepCD47 to PEI-PDT Modified Surfaces
  • 03:21Chandler Loop for Analyzing Cellular Attachment to Metal Surfaces
  • 04:54Analyzing Cellular Attachment to Metal Surfaces using CFDA Dye
  • 05:48Results: Anti-inflammatory Properties of PepCD47 Coated Metal Surfaces Exposed to Whole Blood and Isolated Monocytes
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

此处介绍的是使用多磷酸盐化学将肽 CD47 （pepCD47） 附在金属支架上的规程。使用 pepCD47 使金属支架功能化可防止炎症细胞的附着和激活，从而提高其生物相容性。

Tags

Metal ImplantsCD47Peptide ImmobilizationBiocompatibilityStainless SteelPABTTCEPPEI-PDTPeptide ConjugationCD47 Peptide

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