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Isolering af musenyrnyre resident CD8+ T-celler til flowcytometrianalyse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

Isolering af musenyrnyre resident CD8+ T-celler til flowcytometrianalyse

DOI:
10.3791/61559-v

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06:07 min

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June 27, 2020

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, ,
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Chapters

  • 00:04Introduction
  • 00:56Intravascular Labeling of CD8+ T Cells
  • 02:25Enzymatic Digestion of the Kidney
  • 03:21Density Gradient Centrifugation to Enrich Lymphocytes from the Digested Kidney
  • 04:46Results: Flow Cytometry Analysis of Kidney Lymphocytes
  • 05:40Conclusion

Summary

Automatic Translation

Automatic Translation

Efter virusinfektion, nyre havne et relativt stort antal CD8+ T-celler og giver mulighed for at studere ikke-slimhinde TRM celler. Her beskriver vi en protokol til at isolere mus nyre lymfocytter for flow cytometri analyse.

Tags

Mouse KidneyCD8+ T CellsFlow CytometryBiotin Anti-CD8 AntibodyHomogenization

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