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Isolering af musenyrnyre resident CD8+ T-celler til flowcytometrianalyse
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
DOI:

06:07 min

June 27, 2020

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Chapters

  • 00:04Introduction
  • 00:56Intravascular Labeling of CD8+ T Cells
  • 02:25Enzymatic Digestion of the Kidney
  • 03:21Density Gradient Centrifugation to Enrich Lymphocytes from the Digested Kidney
  • 04:46Results: Flow Cytometry Analysis of Kidney Lymphocytes
  • 05:40Conclusion

Summary

Automatic Translation

Efter virusinfektion, nyre havne et relativt stort antal CD8+ T-celler og giver mulighed for at studere ikke-slimhinde TRM celler. Her beskriver vi en protokol til at isolere mus nyre lymfocytter for flow cytometri analyse.

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