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Konjunkturbestemt fællesskabsisolation og identifikation hos mus
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1Department of Medicine, Division of Infectious Diseases, Brigham and Women’s Hospital,Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School
Chapters
- 00:04Introduction
- 01:02Preparation of Sterile Eye Swabs
- 01:48Workspace Setup
- 02:52Eye Swabbing
- 04:47Enrichment
- 06:02Results: Ocular Microbiome of C57BL/6 Mice
- 07:19Conclusion
Præsenteret her er en protokol for isolering og forstærkning af aerob og fakultativ anaerob mus conjunctival commensal bakterier ved hjælp af en unik øjenpind og kultur-baseret berigelse skridt med efterfølgende identifikation af mikrobiologiske baserede metoder og MALDI-TOF massespektrometri.
