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1Department of Medicine, Division of Infectious Diseases, Brigham and Women’s Hospital,Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School
Chapters
- 00:04Introduction
- 01:02Preparation of Sterile Eye Swabs
- 01:48Workspace Setup
- 02:52Eye Swabbing
- 04:47Enrichment
- 06:02Results: Ocular Microbiome of C57BL/6 Mice
- 07:19Conclusion
Presentert her er en protokoll for isolering og forsterkning av aerob og fakultets anaerob mus konjunktivale kommensale bakterier ved hjelp av en unik øyepinne og kulturbasert berikelse trinn med påfølgende identifikasjon av mikrobiologiske baserte metoder og MALDI-TOF masse spektrometri.
