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Journal / Cancer Research / Isolierung proximaler Flüssigkeiten zur…
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Cancer Research

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JoVE Journal Cancer Research
Isolierung proximaler Flüssigkeiten zur Untersuchung der Tumormikroumgebung des Pankreas-Adenokarzinoms
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Isolierung proximaler Flüssigkeiten zur Untersuchung der Tumormikroumgebung des Pankreas-Adenokarzinoms

Article DOI: 10.3791/61687-v • 05:44 min • November 5th, 2020
November 5th, 2020 •
*1, *2, 1,3, 1,3, 4,5,6, 1,3, 2,7, 2
1Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences, Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, 7Department of Medical Biotechnology and Translational Medicine, University of Milan
* These authors contributed equally

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Pankreassaft ist eine wertvolle Quelle von Biomarkern für menschlichen Bauchspeicheldrüsenkrebs. Wir beschreiben hier eine Methode für das intraoperative Sammelverfahren. Um die Herausforderung zu meistern, dieses Verfahren in Mausmodellen anzuwenden, schlagen wir eine alternative Probe, die interstitielle Tumorflüssigkeit, vor und beschreiben hier zwei Protokolle für ihre Isolierung.

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Krebsforschung Ausgabe 165 Pankreas-Adenokarzinom Pankreassaft Tumor-Interstitialflüssigkeit proximale Flüssigkeit Flüssigbiopsie Biomarker Proteomik
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