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JoVE Journal Cancer Research
Evaluación In Vitro de la Transformación Oncogénica en Células Epiteliales Mamarias Humanas
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Evaluación In Vitro de la Transformación Oncogénica en Células Epiteliales Mamarias Humanas

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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Este protocolo proporciona herramientas experimentales in vitro para evaluar la transformación de las células mamarias humanas. Se describen los pasos detallados para el seguimiento de la tasa de proliferación celular, la capacidad de crecimiento independiente del anclaje y la distribución de linajes celulares en cultivos 3D con matriz de membrana de sótano.

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Investigación del cáncer Número 163 transformación celular curva de crecimiento ensayo de anclaje cultivo celular 3D células epiteliales mamarias humanas tumorigenesis
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