Journal
/
Biology
/
Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

DOI:
10.3791/63585-v

•

07:49 min

•

June 02, 2022

•

, ,
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences,Sun Yat-sen University

Chapters

  • 00:04Introduction
  • 00:47LGSC Primary Culture
  • 03:43LGSC Maintenance and Passage
  • 04:48LGSC Differentiation
  • 05:34Results: Characterization, Differentiation, and Engraftment of LGSCs and Relief of ADDED Symptoms
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

The three-dimensional, serum-free culture method for adult lacrimal gland (LG) stem cells is well established for the induction of LG organoid formation and differentiation into acinar or ductal-like cells.

Tags

Keywords: Lacrimal GlandStem CellsThree-dimensionalSerum-freeCulture SystemRegenerationReconstructionExpansion CapacityDifferentiationEuthanized MouseDispaseCollagenase ITrypsin EDTADulbecco’s Modified Eagles MediumHam’s F-12Matrix GelLacrimal Gland Stem Cell Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63585?article_type=v

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
check_url/63585?article_type=v

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
check_url/63585?article_type=v

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
check_url/63585?article_type=v

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
check_url/63585?article_type=v

Isolation of Mouse Salivary Gland Stem Cells
check_url/63585?article_type=v

Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
check_url/63585?article_type=v

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
check_url/63585?article_type=v

Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
check_url/63585?article_type=v

Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
check_url/63585?article_type=v

Manipulating the Murine Lacrimal Gland

Read Article