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使用三维共培养系统NICO-1评估卵巢癌干细胞样细胞的血管遗传特性
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Biology
Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
Please note that all translations are automatically generated.
Click here for the English version.
使用三维共培养系统NICO-1评估卵巢癌干细胞样细胞的血管遗传特性
DOI:
10.3791/61751-v
•
07:59 min
•
December 05, 2020
•
Yuko Miyagawa
,
Kazunori Nagasaka
,
Kaoru Yamawaki
3
,
Yutaro Mori
3
,
Tatsuya Ishiguro
,
Kei Hashimoto
,
Ryoko Koike
,
Siho Fukui
,
Takeru Sugihara
,
Takayuki Ichinose
,
Haruko Hiraike
,
Koichiro Kido
,
Koji Okamoto
,
Takayuki Enomoto
,
Takuya Ayabe
1
Department of Obstetrics and Gynecology
,
Teikyo University School of Medicine
,
2
Department of Obstetrics and Gynecology
,
Niigata University Graduate School of Medical and Dental Sciences
,
3
Devision of Cancer Differentiation
,
National Cancer Center Research Institute
Chapters
00:05
Introduction
00:55
Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
02:23
CSC Passage
03:24
HUEhT-1 Endothelial Cell Culture
04:14
NICO-1 Coculture Plate Preparation
05:16
NICO-1 System HUEhT-1 and CSC Seeding
06:14
Results: Representative CSC-Induced Neovascularization and Vascular Formation
07:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
卵巢癌干细胞 (OCSC) 负责癌症的发生、复发、治疗耐药性和转移。OCSC血管生态位被认为可促进OCSC的自我更新,导致化学耐药性。该协议为体外建立可重复的OCSC血管生态位模型提供了基础
。
Tags
Ovarian Cancer
Cancer Stem Cells
Angiogenesis
3D Co-culture System
NICO-1
Density Gradient Centrifugation
OCSC Medium
HUEhT-1 Endothelial Cells
Tumor Microenvironment
Paracentesis
Cell Detachment
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