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Journal
/
Developmental Biology
/
초기
초파리
배아 내의 면역조직화학 및 RNA
계
내 분포를 위한 프로토콜
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
A Protocol for Immunohistochemistry and RNA
In-situ
Distribution within Early
Drosophila
Embryo
Please note that all translations are automatically generated.
Click here for the English version.
초기
초파리
배아 내의 면역조직화학 및 RNA
계
내 분포를 위한 프로토콜
DOI:
10.3791/61776-v
•
14:34 min
•
May 06, 2022
•
Wei Zhang*
1
,
Xinjuan Lei*
1
,
Xin Zhou*
2,3
,
Boling He
,
Liqin Xiao
,
Huimin Yue
,
Shulin Wang
,
Yuting Sun
,
Yajun Wu
,
Liyang Wang
4
,
George Ghartey-Kwansah
,
Odell D. Jones
,
Joseph L. Bryant
,
MengMeng Xu
,
Jianjie Ma
,
Xuehon Xu
1
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB
,
Shaanxi Normal University College of Life Sciences
,
2
Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine
,
Xi’an Medical University
,
3
Ohio State University College of Medicine
,
4
Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center
,
Harvard Medical School
,
5
University of Pennsylvania ULAR
,
6
University of Maryland School of Medicine
,
7
Columbia University Medical Center
Chapters
00:05
Introduction
00:44
Coating Slides
01:41
Embedding of
Drosophila
Embryos
04:25
Hematoxylin-Eosin Staining
05:25
Periodic Acid-Silver Methenamine Staining
06:28
Immunohistochemistry
08:15
In Situ Hybridization
12:17
Results
13:32
Discussion
Summary
Automatic Translation
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Automatic Translation
여기에서, 우리는
초파리
배아 단백질 및 RNA의 검출 및 국소화를 위한 프로토콜을 기술한다 수집에서 사전-포베딩 및 포매, 면역염색, 및 mRNA
를 계내
혼성화에 이르게 한다.
Tags
Keywords: Drosophila
Embryo
Immunohistochemistry
RNA In-situ
Histochemistry
Protein Localization
Developmental Biology
Chorion
Gelatin Coating
Bleach Dechorionation
N-heptane
Methanol Precipitation
Article
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