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Journal / Developmental Biology / 초기 초파리 배아 내의 면역조직화학 및 RNA 계내 분포를 위한…
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Developmental Biology
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JoVE Journal Developmental Biology
초기 초파리 배아 내의 면역조직화학 및 RNA 계내 분포를 위한 프로토콜
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초기 초파리 배아 내의 면역조직화학 및 RNA 계내 분포를 위한 프로토콜

Article DOI: 10.3791/61776-v • 14:34 min • May 6th, 2022
May 6th, 2022 •
*1, *1, *2,3, 1, 1, 1, 1, 1, 1, 1,4, 1, 5, 6, 7, 3, 1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
* These authors contributed equally

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여기에서, 우리는 초파리 배아 단백질 및 RNA의 검출 및 국소화를 위한 프로토콜을 기술한다 수집에서 사전-포베딩 및 포매, 면역염색, 및 mRNA 를 계내 혼성화에 이르게 한다.

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발달 생물학 문제 183 초파리 배아 파라핀 섹션 코팅 슬라이드 임베딩 현장 혼성화 DmFKBP12 / Calstabin
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