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Een adipocytencelkweekmodel om de impact van eiwit- en micro-RNA-modulatie op de adipocytenfunctie te bestuderen
JoVE Journal
Biology
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JoVE Journal Biology
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
DOI:

09:20 min

May 04, 2021

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Chapters

  • 00:05Introduction
  • 00:41Differentiation of Murine 3T3-L1 Fibroblasts into Adipocytes and Preparation of Precoated Plates
  • 03:18Preparation of the Transfection Mix and 3T3-L1 Adipocytes
  • 06:28Results: Differentiation, Protein Silencing, Micro-RNA Overexpression and Effects of Protein or Micro-RNA Modulation in 3T3-L1 Adipocytes
  • 08:35Conclusion

Summary

Automatic Translation

Hier wordt een protocol gepresenteerd om oligonucleotiden zoals klein-interfererend RNA (siRNA), micro-RNA-mimics (miRs) of anti-micro-RNA (anti-miR) af te leveren in volwassen adipocyten om eiwit- en micro-RNA-expressie te moduleren.

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