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Biochemistry
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HPLC-MS/MSによるヌクレオシド/ヌクレオチド含有量測定のための植物サンプル調製
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JoVE Journal
Biochemistry
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Please note that all translations are automatically generated.
Click here for the English version.
HPLC-MS/MSによるヌクレオシド/ヌクレオチド含有量測定のための植物サンプル調製
DOI:
10.3791/61956-v
•
06:38 min
•
February 24, 2021
•
Changhua Zhu*
1
,
Xiaoye Liu*
2
,
Wenlei Wang
,
Xiaoguang Chen
,
Shangyu Gao
,
Meng Qian
,
Na Yang
,
Yifeng Xu
,
Mingjia Chen
1
College of Life Sciences
,
Nanjing Agricultural University
,
2
Department of Criminal Science and Technology
,
Nanjing Forest Police College
,
3
Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition
,
Leibniz University Hannover
Chapters
00:04
Introduction
00:54
Nucleosides/Nucleotides Extraction
02:57
LC-MS/MS Measurement
04:27
Results: Identification of N1-Methyladenosine by Mass Transition
05:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
インビボヌクレオシド/ヌクレオチドの植物定量の正確かつ再現可能な方法をここに記載する。この方法では、HPLC-MS/MS を採用しています。
Tags
Plant Sample Preparation
Nucleosides
Nucleotides
HPLC-MS/MS
Metabolite Extraction
Arabidopsis
LC-MS/MS
Ammonium Acetate Buffer
Methanol
Acetonitrile
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