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Planteprøveforberedelse for nukleoside/nukleotidinnholdsmåling med en HPLC-MS/MS
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
DOI:

06:38 min

February 24, 2021

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Chapters

  • 00:04Introduction
  • 00:54Nucleosides/Nucleotides Extraction
  • 02:57LC-MS/MS Measurement
  • 04:27Results: Identification of N1-Methyladenosine by Mass Transition
  • 05:57Conclusion

Summary

Automatic Translation

En presis og reproduserbar metode for in vivo nukleosider/nukleotider kvantifisering i planter er beskrevet her. Denne metoden bruker en HPLC-MS/MS.

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