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Cardiac Sfæroider som in vitro Bioengineered hjertevæv at studere Human Heart Patofysiologi
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Cardiac Sfæroider som in vitro Bioengineered hjertevæv at studere Human Heart Patofysiologi

DOI:
10.3791/61962-v

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10:41 min

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January 23, 2021

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,
1University of Newcastle, 2University of Sydney, 3Kolling Institute of Medical Research,Royal North Shore Hospital, 4University of Technology, Sydney

Chapters

  • 00:00Introduction
  • 00:54Cardiac Spheroid Formation
  • 05:46Drug Testing
  • 06:42Representative Results
  • 07:36Endothelial Cell Network Staining
  • 09:52Representative Results
  • 10:04Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol har til formål at fremstille 3D hjerte sfæroider (CSs) ved co-dyrkning celler i hængende dråber. Kollagen-indlejrede CS'er behandles med doxorubicin (DOX, et kardiotoksisk middel) ved fysiologiske koncentrationer for at modellere hjertesvigt. In vitro-test ved hjælp af DOX-behandlede CS'er kan anvendes til at identificere nye behandlinger for hjertesvigt patienter.

Tags

Cardiac SpheroidsIn VitroHuman Heart PathophysiologyCardiac MyocytesCardiac FibroblastsCardiac Endothelial CellsDrug Toxicity AssaysDoxorubicinCardiomyocytesCell CultureHanging DropSpheroid Formation

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