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通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
DOI:

04:30 min

February 04, 2021

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Chapters

  • 00:05Introduction
  • 00:30Cloning Phase
  • 01:48T Lymphocyte Culture
  • 02:52Results: Representative Calcified Aortic Valve T Cell Isolation and Analysis
  • 03:52Conclusion

Summary

Automatic Translation

在本研究中,我们描述了从钙化主动脉瓣新鲜样品中分离T淋巴细胞的过程,以及使用流式细胞术分析表征适应性白细胞亚群的T细胞克隆的分析步骤。

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