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通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
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通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化

DOI:
10.3791/62059-v

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04:30 min

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February 04, 2021

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, , , , , ,
1Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine,University of Florence, 3Berlin Institute of Health, 4Department of Cardiology,German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology,Helios Klinikum Erfurt

Chapters

  • 00:05Introduction
  • 00:30Cloning Phase
  • 01:48T Lymphocyte Culture
  • 02:52Results: Representative Calcified Aortic Valve T Cell Isolation and Analysis
  • 03:52Conclusion

Summary

Automatic Translation

Automatic Translation

在本研究中，我们描述了从钙化主动脉瓣新鲜样品中分离T淋巴细胞的过程，以及使用流式细胞术分析表征适应性白细胞亚群的T细胞克隆的分析步骤。

Tags

T LymphocytesAortic Valve CalcificationFeeder CellsDensity Gradient CentrifugationCell CulturePBMC IsolationIL-2T-cell CloningT-cell CultureU-bottom 96-well Plates

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