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Undersøgelse af aortaklappen Forkalkning via isolation og kultur af T-lymfocytter ved hjælp af feederceller fra bestrålet Buffy Coat
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Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

Undersøgelse af aortaklappen Forkalkning via isolation og kultur af T-lymfocytter ved hjælp af feederceller fra bestrålet Buffy Coat

DOI:
10.3791/62059-v

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04:30 min

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February 04, 2021

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, , , , , ,
1Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine,University of Florence, 3Berlin Institute of Health, 4Department of Cardiology,German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology,Helios Klinikum Erfurt

Chapters

  • 00:05Introduction
  • 00:30Cloning Phase
  • 01:48T Lymphocyte Culture
  • 02:52Results: Representative Calcified Aortic Valve T Cell Isolation and Analysis
  • 03:52Conclusion

Summary

Automatic Translation

Automatic Translation

I denne undersøgelse beskriver vi processen med T-lymfocytisolation fra friske prøver af forkalkede aortaventiler og de analytiske trin i T-cellekloning til karakterisering af de adaptive leukocytundersæt ved hjælp af flowcytometrianalyse.

Tags

T LymphocytesAortic Valve CalcificationFeeder CellsDensity Gradient CentrifugationCell CulturePBMC IsolationIL-2T-cell CloningT-cell CultureU-bottom 96-well Plates

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