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Chapters
- 00:04Introduction
- 00:56Epithelial Monolayer Preparation
- 06:10TEER Measurement
- 07:05Results: Analysis of Epithelial Organoid Monolayers
- 09:06Conclusion
Here, we describe the preparation of human organoid-derived intestinal epithelial monolayers for studying intestinal barrier function, permeability, and transport. As organoids represent original epithelial tissue response to external stimuli, these models combine the advantages of expandability of cell lines and the relevance and complexity of primary tissue.
