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Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
JoVE Journal
Biology
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JoVE Journal Biology
Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
DOI:

09:40 min

July 29, 2021

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Chapters

  • 00:04Introduction
  • 00:56Epithelial Monolayer Preparation
  • 06:10TEER Measurement
  • 07:05Results: Analysis of Epithelial Organoid Monolayers
  • 09:06Conclusion

Summary

Automatic Translation

Here, we describe the preparation of human organoid-derived intestinal epithelial monolayers for studying intestinal barrier function, permeability, and transport. As organoids represent original epithelial tissue response to external stimuli, these models combine the advantages of expandability of cell lines and the relevance and complexity of primary tissue.

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