A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 01:02Isolation of Rat/Mouse and Rabbit PE Cells
- 03:35Isolation of Pig PE Cells
- 04:40Isolation of Bovine PE Cells
- 06:58Cultivation – Medium Change
- 07:39Results: Isolation, Culture, and Transfection of Primary Pigment Epithelial (PE) Cells from Different Mammalian Species
- 09:04Conclusion
Here, a protocol to isolate and transfect primary iris and retinal pigment epithelial cells from various mammals (mice, rat, rabbit, pig, and bovine) is presented. The method is ideally suited to study ocular gene therapy approaches in various set-ups for ex vivo analyses and in vivo studies transferable to humans.
