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Journal / Genetics / Integrazione mediata dal sito φC31 e scambio di…
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Genetics

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JoVE Journal Genetics
Integrazione mediata dal sito φC31 e scambio di cassette in Anopheles Vettori della malaria
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Integrazione mediata dal sito φC31 e scambio di cassette in Anopheles Vettori della malaria

Article DOI: 10.3791/62146-v • 09:38 min • February 2nd, 2021
February 2nd, 2021 •
1,2, 1, 1, 2,3
1Vector Biology Department, Liverpool School of Tropical Medicine, 2Department of Microbiology & Molecular Genetics, University of California, 3Department of Molecular Biology & Biochemistry, University of California

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Il protocollo descrive come ottenere modifiche dirette al sito nel genoma delle zanzare Anopheles malaria utilizzando il sistema φC31 . Le modifiche descritte includono sia l'integrazione che lo scambio di cassette transgeniche nel genoma di linee di attP..

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Genetica Numero 168 Anopheles φC31 site-directed docking integrazione scambio di cassette zanzare transgenico
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