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Journal / Developmental Biology / Generatie en uitbreiding van menselijke…
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JoVE Journal Developmental Biology
Generatie en uitbreiding van menselijke cardiomyocyten uit mononucleaire bloedcellen van de patiënt
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Generatie en uitbreiding van menselijke cardiomyocyten uit mononucleaire bloedcellen van de patiënt

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Hier presenteren we een protocol om menselijke cardiomyocyten robuust te genereren en uit te breiden uit perifere mononucleaire bloedcellen van patiënten.

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Ontwikkelingsbiologie perifere mononucleaire bloedcellen PBMC's door de mens geïnduceerde pluripotente stamcellen iPSC's cardiale differentiatie iPSC-herprogrammering cardiomyocytenexpansie.
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