JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
患者末梢血単核細胞からのヒト心筋細胞の発生と拡大
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells
Please note that all translations are automatically generated.
Click here for the English version.
患者末梢血単核細胞からのヒト心筋細胞の発生と拡大
DOI:
10.3791/62206-v
•
05:38 min
•
February 12, 2021
•
Shiqiao Ye
2
,
Xiaoping Wan
,
Juan Su
2
,
Akshar Patel
2,4
,
Blake Justis
2,5
,
Isabelle Deschênes
,
Ming-Tao Zhao
2,5,6
1
Center for Cardiovascular Research, The Abigail Wexner Research Institute
,
Nationwide Children’s Hospital
,
2
The Heart Center
,
Nationwide Children’s Hospital
,
3
Department of Physiology and Cell Biology
,
The Ohio State University College of Medicine
,
4
Department of Anatomy
,
The Ohio State University College of Medicine
,
5
MCDB Graduate Program
,
The Ohio State University
,
6
Department of Pediatrics
,
The Ohio State University College of Medicine
Chapters
00:04
Introduction
01:08
Human Induced Pluripotent Stem Cell (iPSC) Maintenance and Passaging
01:55
Chemically Defined Cardiomyocyte (CM) Differentiation
02:57
Results: Representative iPSC-CM Differentiation and Function
04:51
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、患者末梢血単核細胞からヒト心筋細胞を強固に生成・拡張するプロトコルを提示する。
Tags
Cardiomyocyte Generation
Peripheral Blood Mononuclear Cells
IPSC Reprogramming
Cardiovascular Disease Modeling
Cardiac Toxicity Testing
Differentiation Protocol
Cell Culture
Sendai Virus
PBMC
E8 Medium
Cardiomyocyte Differentiation Media
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
末梢血造血前駆細胞からヒト神経幹細胞の直接誘導
フィーダーフリー条件にセンダイウイルスを用いてヒト末梢T細胞から人工多能性幹細胞の生成
単離およびヒト胎盤組織由来の間葉系幹/間質細胞の増殖
再現性の高い小分子ベースの分化プロトコルを使用したヒト多能性幹細胞から心筋細胞の大規模生産
センダイウイルスや遠心分離を用いて血液細胞から誘導多能性幹細胞の生成
エピソームベクトルを用いたヒト末梢血単核細胞からの統合フリー人工多能性幹細胞の生成
ヒト多能性幹細胞から肝細胞様細胞の定義され、スケーラブルな世代
末梢血由来ヒト誘導多能性幹細胞からの軟骨細胞の分化
ひと臍帯血由来の血管内皮前駆細胞の分離
最初心臓フィールドのような心臓前駆細胞およびひと多能性幹細胞からのような心室心筋細胞の生成
Read Article