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ヒト骨格筋微小組織における骨格筋の健康の機能指標の評価
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
DOI:

09:30 min

February 18, 2021

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Chapters

  • 00:04Introduction
  • 00:42Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
  • 02:51Preparation of Cells for hMMTs Seeding
  • 05:24Seeding hMMTs
  • 07:24Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
  • 08:57Conclusion

Summary

Automatic Translation

本稿は、収縮力やカルシウム処理分析を含む機能の機能アッセイにおいて、3Dヒト骨格筋微小組織および低侵襲下流部のアレイを生成するための詳細なプロトコルを記述する。

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