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Bioengineering
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ヒト骨格筋微小組織における骨格筋の健康の機能指標の評価
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
Please note that all translations are automatically generated.
Click here for the English version.
ヒト骨格筋微小組織における骨格筋の健康の機能指標の評価
DOI:
10.3791/62307-v
•
09:30 min
•
February 18, 2021
•
Heta Lad
2
,
Brennen Musgrave
2
,
Majid Ebrahimi
2
,
Penney M. Gilbert
2,3
1
Institute of Biomedical Engineering
,
University of Toronto
,
2
Donnelly Centre for Cellular and Biomolecular Research
,
University of Toronto
,
3
Department of Cell and Systems Biology
,
University of Toronto
Chapters
00:04
Introduction
00:42
Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
02:51
Preparation of Cells for hMMTs Seeding
05:24
Seeding hMMTs
07:24
Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
08:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
本稿は、収縮力やカルシウム処理分析を含む機能の機能アッセイにおいて、3Dヒト骨格筋微小組織および低侵襲下流部のアレイを生成するための詳細なプロトコルを記述する。
Tags
Muscle Biology
Disease Modeling
Candidate Molecule Testing
Contractile Force
Calcium Transients
Longitudinal Studies
Basement Membrane Extract
Thrombin
Fibrinogen
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