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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Article DOI: 10.3791/62431-v • 09:03 min • July 19th, 2021
July 19th, 2021 •
1, 1, 2, 2, 1, 1
1Medical Mycology Research Center, Chiba University, 2Marine Works Japan Ltd.

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Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.

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Rapid Freezing Sandwich Freezing Device Ultrastructural Preservation Biological Specimens Electron Microscopy Freeze-substituted Specimens Liquid Propane Liquid Nitrogen Copper Discs Glow Discharge Hydrophilic Surface Cell Suspension
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