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单骨骼肌肉纤维解剖，用于全山神经肌肉结的免疫荧光和形态分析

JoVE Journal
Neuroscience
Author Produced

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JoVE Journal Neuroscience

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

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单骨骼肌肉纤维解剖，用于全山神经肌肉结的免疫荧光和形态分析

DOI:

10.3791/62620-v

•

08:41 min

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August 14, 2021

•

Carmen Bolatto*¹, Silvia Olivera-Bravo*², Sofía Cerri²

¹Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine,Universidad de la República, ²Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE),Ministerio de Educación y Cultura

Chapters

00:17Introduction
00:45Muscle Dissection
04:43Teased Fiber Preparation
06:44Results: Morphometric Evaluation of Whole-Mount Neuromuscular Junctions
08:06Conclusion

Summary

Automatic Translation

准确检测神经肌肉结状组件的能力对于评估其结构中的修改至关重要，因为病理学或发育过程。在这里，我们提出了一个简单明了的方法，以获得全安装神经肌肉交汇点的高质量图像，可用于执行定量测量的完整描述。

Tags

Skeletal Muscle Fibers Immunofluorescent Morphometric Analyses Whole-mount Neuromuscular Junctions EDL Muscle Soleus Muscle Dissection Surgical Instruments Fixation

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