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Journal
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Bioengineering
/
一锅纯无细胞系统
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
OnePot PURE Cell-Free System
Please note that all translations are automatically generated.
Click here for the English version.
一锅纯无细胞系统
DOI:
10.3791/62625-v
•
08:25 min
•
June 23, 2021
•
Laura Grasemann*
1
,
Barbora Lavickova*
1
,
M. Carolina Elizondo-Cantú
,
Sebastian J. Maerkl
1
Institute of Bioengineering, School of Engineering
,
École Polytechnique Fédérale de Lausanne
Chapters
00:04
Introduction
00:54
OnePot Protein Solution Expression and Purification
05:44
OnePot PURE Reaction
06:51
Results: OnePot Protein Purification and Overexpression
07:44
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种使用标准实验室设备生产重组纯纯无细胞TX-TL系统的快速且经济高效的方法。
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Cell-free Protein Expression
PURE System
OnePot Method
Recombinant Protein Purification
Streamlined Protein Production
Time-efficient System
Cost-effective Platform
Synthetic Cell-free Biology
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