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Reconstruct Human Retinoblastoma In Vitro
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JoVE Journal Cancer Research
Reconstruct Human Retinoblastoma In Vitro

Reconstruct Human Retinoblastoma In Vitro

DOI:
10.3791/62629-v

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06:52 min

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October 11, 2022

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1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital,Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

Chapters

  • 00:04Introduction
  • 00:46Generation of Human Retinoblastoma
  • 04:39Results: In Vitro Reconstruction of Human Retinoblastoma
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish.

Tags

Keywords: RetinoblastomaIn VitroHuman Embryonic Stem CellsRB1 KnockoutRetinal Cell DifferentiationDispase BufferGrowth Factor Reduced Basement Membrane MatrixMedium 1Cyst Formation

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