Journal
/
Immunology and Infection
/
使用类似人类单细胞的THP-1白血病细胞系，将巨噬细胞分化和极化成M2样表型
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
Please note that all translations are automatically generated. Click here for the English version.

使用类似人类单细胞的THP-1白血病细胞系，将巨噬细胞分化和极化成M2样表型

DOI:
10.3791/62652-v

•

06:38 min

•

August 02, 2021

•

, , , ,
1Price Institute of Surgical Research, Department of Surgery,University of Louisville

Chapters

  • 00:05Introduction
  • 00:56Culturing and Maintenance of THP‐1 Monocyte-like Cells
  • 02:59Seeding of THP‐1 cells and Differentiation into M0 Macrophages
  • 04:01Polarization of M0 Macrophages into M2‐like Macrophages
  • 04:39Detaching and Harvesting Macrophages for Flow Cytometry
  • 05:14Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

M2样肿瘤相关巨噬细胞（TAM）与肿瘤进展和癌症预后不佳有关。本协议作为在 14 天内可重复区分和分化 THP-1 单细胞状细胞成 M2 样巨噬细胞的详细指南。该模型是研究TAM在肿瘤微环境内的抗炎作用的基础。

Tags

MacrophageDifferentiationPolarizationM2-like PhenotypeTHP-1 CellsAnti-inflammatoryTumor-associated MacrophagesColon CancerCell CultureCell CountingCell Viability

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Antileishmanial筛选对细胞内的寄生虫救援和转化实验<em>杜氏利什曼原虫</em无鞭毛体在THP1人急性单核细胞白血病细胞株

使用骨髓源性巨噬细胞巨噬细胞极化的调查

一个<em在体外</em>模型研究人类巨噬细胞分化和极化的异质性

建立一个<em>在体外</em>系统来研究细胞内的行为<em>光滑念珠菌</em>在人THP-1巨噬细胞

评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。

人THP-1巨噬细胞通过核转高效转染

鼠标朴素CD4<sup> +</sup> T细胞分离和<em>在体外</em>分化为T细胞亚群

利用共焦显微镜可视化巨噬细胞胞外陷阱

一种研究人体 T 细胞召回反应的吸水泡协议

巨细胞动脉炎叶酸受体贝塔巨噬细胞和血管免疫微环境的免疫组织病理学分析

Read Article