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Makrofag differentiering og polarisering i en M2-lignende fænotype ved hjælp af en human monocyt-lignende THP-1 Leukæmi Cell Line
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Chapters
- 00:05Introduction
- 00:56Culturing and Maintenance of THP‐1 Monocyte-like Cells
- 02:59Seeding of THP‐1 cells and Differentiation into M0 Macrophages
- 04:01Polarization of M0 Macrophages into M2‐like Macrophages
- 04:39Detaching and Harvesting Macrophages for Flow Cytometry
- 05:14Results: Macrophage Differentiation and Polarization into an M2‐Like Phenotype
- 05:56Conclusion
M2-lignende tumor-associerede makrofager (TAM) er forbundet med tumor progression og dårlig prognose i kræft. Denne protokol fungerer som en detaljeret guide til reproducerer differentiere og polarisere THP-1 monocyt-lignende celler i M2-lignende makrofager inden for 14 dage. Denne model er grundlaget for at undersøge de antiinflammatoriske virkninger af TAM inden for tumormikromiljøet.
