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Developmental Biology

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Nuclear Migration in the Drosophila Oocyte

Nuclear Migration in the Drosophila Oocyte

Article DOI: 10.3791/62688-v
May 13th, 2021


Summary May 13th, 2021

In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.


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