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用于工程神经样组织的小鼠皮质星形胶质细胞的3D生物打印
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue
Please note that all translations are automatically generated.
Click here for the English version.
用于工程神经样组织的小鼠皮质星形胶质细胞的3D生物打印
DOI:
10.3791/62691-v
•
08:57 min
•
July 16, 2021
•
Bruna A. G. de Melo
,
Elisa M. Cruz
,
Taís N. Ribeiro
,
Mayara V. Mundim
,
Marimelia A. Porcionatto
1
Department of Biochemistry, Escola Paulista de Medicina
,
Universidade Federal de São Paulo
Chapters
00:05
Introduction
00:49
Astrocyte Isolation and Culture
02:06
Astrocytes-laden Gelatin/GelMA/Fibrinogen Bioink Preparation
04:01
Bioprinting Syringe Preparation and Bioprinting
04:58
Bioprinted Construct and Culture Crosslinking
05:40
Astrocytes Viability Assessment
06:22
Results: 3D Bioprinted Astrocyte Cell Viability and Morphology Assessment
08:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们报道了一种3D生物打印小鼠皮质星形胶质细胞的方法,用于生物制造神经样组织,以研究星形胶质细胞在中枢神经系统中的功能以及涉及神经胶质细胞在神经系统疾病和治疗中的机制。
Tags
3D Bioprinting
Astrocytes
In Vitro Models
Neurological Disease
Biomaterials
Extracellular Matrix
Fibrinogen
Gelatin
Photo-initiator
Laminin
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