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Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
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Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

DOI:
10.3791/62778-v

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11:30 min

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July 24, 2021

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, , ,
1Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications,National Centre for Scientific Research, Demokritos (NCSRD), 2Light Microscopy Unit, Institute of Biosciences and Applications,National Centre for Scientific Research, Demokritos (NCSRD)

Chapters

  • 00:15Introduction
  • 01:49Inoculum Preparation
  • 03:48Preparation of Samples for Imaging Filamentous Fungal Cells Grown on Solid Medium
  • 05:04Preparation of Samples for Imaging Filamentous Fungal Cells Grown on Liquid Medium
  • 05:41Live-Cell Microscopy
  • 06:52Image Analysis
  • 09:28Representative Results
  • 10:55Conclusion

Summary

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We present a label-free live imaging protocol using transmitted light microscopy techniques to capture images, analyze and quantify growth kinetics of the filamentous fungus A. nidulans in both submerged cultures and solid media. This protocol can be used in conjunction with fluorescence microscopy.

Tags

Keywords: Aspergillus NidulansLive MicroscopyOpen-source SoftwareQuantitative AnalysisGrowth RateFilamentous FungiTime-lapse MicroscopyColony DiameterSingle-cell MeasurementsLiquid CultureAgar MediumConidiaSporesTween 80Inverted Agar Method

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