Journal
/
Biology
/
Kvantitativ analyse af Aspergillus nidulans vækstrate ved hjælp af Live Microscopy og Open Source Software
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Kvantitativ analyse af Aspergillus nidulans vækstrate ved hjælp af Live Microscopy og Open Source Software

DOI:
10.3791/62778-v

•

11:30 min

•

July 24, 2021

•

, , ,
1Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications,National Centre for Scientific Research, Demokritos (NCSRD), 2Light Microscopy Unit, Institute of Biosciences and Applications,National Centre for Scientific Research, Demokritos (NCSRD)

Chapters

  • 00:15Introduction
  • 01:49Inoculum Preparation
  • 03:48Preparation of Samples for Imaging Filamentous Fungal Cells Grown on Solid Medium
  • 05:04Preparation of Samples for Imaging Filamentous Fungal Cells Grown on Liquid Medium
  • 05:41Live-Cell Microscopy
  • 06:52Image Analysis
  • 09:28Representative Results
  • 10:55Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en etiketfri live imaging protokol ved hjælp af transmitterede lysmikroskopi teknikker til at fange billeder, analysere og kvantificere vækst kinetik af filamentous svamp A. nidulans i både nedsænket kulturer og faste medier. Denne protokol kan bruges sammen med fluorescensmikroskopi.

Tags

Aspergillus NidulansLive MicroscopyOpen-source SoftwareQuantitative AnalysisGrowth RateFilamentous FungiTime-lapse MicroscopyColony DiameterSingle-cell MeasurementsLiquid CultureAgar MediumConidiaSporesTween 80Inverted Agar Method

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Kvantitative levende celler fluorescens-mikroskopi Analyse af Fission Yeast

Kvantitativ analyse af Random migration af celler Brug af Time-lapse Video Microscopy

Kvantitativ analyse af Autophagy hjælp Advanced 3D fluorescensmikroskopi

Workflow til High-indhold, individuel celle Kvantificering af fluorescerende markører fra Universal Microscope data, støttet af Open Source Software

Brug plusTipTracker Software til måling mikrotubulære Dynamics i<em> Xenopus laevis</em> vækstkegler

Sporing og kvantificere udviklingsprocesser i<em> C. elegans</em> Brug Open source Værktøj

Open Source High Content Analysis hjælp Automated Fluorescens Lifetime Imaging Microscopy

Open source Single-partikel Analyse for Super-resolution Microscopy med VirusMapper

Read Article