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Journal / Bioengineering / Cultivo microbiano automatizado y evolución…
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JoVE Journal Bioengineering
Cultivo microbiano automatizado y evolución adaptativa utilizando el sistema de cultivo de microgotas microbianas (MMC)
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Cultivo microbiano automatizado y evolución adaptativa utilizando el sistema de cultivo de microgotas microbianas (MMC)

Article DOI: 10.3791/62800-v • 08:18 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1,2,3, 4, 1,2,6, 1,2,5, 3, 1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
* These authors contributed equally

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Este protocolo describe cómo utilizar el sistema de cultivo microbiano de microgotas (MMC) para llevar a cabo el cultivo microbiano automatizado y la evolución adaptativa. MMC puede cultivar y subcultivar microorganismos de forma automática y continua y monitorear en línea su crecimiento con un rendimiento relativamente alto y una buena paralelización, reduciendo el consumo de mano de obra y reactivos.

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Bioingeniería Número 180 sistema de cultivo de microgotas microbianas operaciones automatizadas alto rendimiento cultivo microbiano evolución adaptativa de laboratorio detección en línea
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