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JoVE Journal Bioengineering
Konstruksjon av modell lipidmembraner som inkorporerer G-proteinkoblede reseptorer (GPCRs)
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Konstruksjon av modell lipidmembraner som inkorporerer G-proteinkoblede reseptorer (GPCRs)

Article DOI: 10.3791/62830-v • 09:45 min • February 5th, 2022
February 5th, 2022 •
*1, *1, 1
1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California
* These authors contributed equally

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Denne protokollen benytter agarose hevelse som en kraftig og generaliserbar teknikk for å inkorporere integrerte membranproteiner (IMPs) i gigantiske unilamellar lipid vesicles (GUVs), som beskrevet her for rekonstituering av humant 1A serotoninreseptorprotein (5-HT1AR), en av klassene av farmakologisk viktige G protein-koblede reseptorer.

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