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Konstruktion af Model Lipid Membraner indarbejde G-protein koblede receptorer (GPCRs)
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Chapters
- 00:05Introduction
- 01:16Preparation of Materials and Reagents
- 02:58Hydrogel Preparation
- 03:40Agarose and Protein Mixing
- 04:05Hydrogel and Lipid Deposition
- 06:09Swelling and Harvesting of Vesicles
- 08:03Representative Results: Microscopy of Vesicles
- 08:51Conclusion
Denne protokol udnytter agarose hævelse som en kraftfuld og generaliserbar teknik til at indarbejde integrerede membranproteiner (IMP) i gigantiske unilamellar lipid vesikler (GUVs), som beskrevet her for rekonstitution af human 1A serotonin receptor protein (5-HT1AR), en af klasserne af farmakologisk vigtige G protein-koblede receptorer.
