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Journal / Bioengineering / Konstruktion af Model Lipid Membraner indarbejde…
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JoVE Journal Bioengineering
Konstruktion af Model Lipid Membraner indarbejde G-protein koblede receptorer (GPCRs)
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Konstruktion af Model Lipid Membraner indarbejde G-protein koblede receptorer (GPCRs)

Article DOI: 10.3791/62830-v • 09:45 min • February 5th, 2022
February 5th, 2022 •
*1, *1, 1
1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California
* These authors contributed equally

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Denne protokol udnytter agarose hævelse som en kraftfuld og generaliserbar teknik til at indarbejde integrerede membranproteiner (IMP) i gigantiske unilamellar lipid vesikler (GUVs), som beskrevet her for rekonstitution af human 1A serotonin receptor protein (5-HT1AR), en af klasserne af farmakologisk vigtige G protein-koblede receptorer.

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