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JoVE Journal Bioengineering
Construction de membranes lipidiques modèles incorporant des récepteurs couplés aux protéines G (RCPG)
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Construction de membranes lipidiques modèles incorporant des récepteurs couplés aux protéines G (RCPG)

Article DOI: 10.3791/62830-v • 09:45 min • February 5th, 2022
February 5th, 2022 •
*1, *1, 1
1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California
* These authors contributed equally

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Ce protocole utilise le gonflement de l’agarose comme une technique puissante et généralisable pour incorporer des protéines membranaires intégrales (PIM) dans des vésicules lipidiques unilamellaires géantes (GUV), comme décrit ici pour la reconstitution de la protéine réceptrice de la sérotonine 1A humaine (5-HT1AR), l’une des classes de récepteurs couplés à la protéine G pharmacologiquement importants.

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Bioingénierie numéro 180
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