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Høj-gennemløb, robust og meget tidsfleksibel metode til overfladesterilisering af Arabidopsis Frø
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JoVE Journal Biology
High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

Høj-gennemløb, robust og meget tidsfleksibel metode til overfladesterilisering af Arabidopsis Frø

DOI:
10.3791/62893-v

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07:28 min

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October 04, 2021

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, , ,
1Department of Biodiversity and Molecular Ecology, Research and Innovation Centre,Fondazione Edmund Mach

Chapters

  • 00:04Introduction
  • 00:46Reagents and Media Preparation
  • 01:59Aspirator Setup
  • 02:37High-Throughput Liquid Surface Sterilization of Seeds
  • 04:56Plating and Scoring of Arabidopsis on Half Strength Murashige and Skoog Plates
  • 05:32Results: Germination Analysis of Arabidopsis Seeds After Surface Sterilization with the New Method
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

En højgennemsigtsprotokol til overfladesterilisering af Arabidopsisthaliana (Arabidopsis) frø leveres, hvilket optimerer væskehåndteringstrinnene med en simpel sugeenhed konstrueret med en vakuumpumpe. Hundredvis af frøprøver kan overfladesteriliseres på en dag.

Tags

ArabidopsisSeed Surface SterilizationHigh-throughputRobustTime-flexibleFunctional GenomicsModel Plant SpeciesEthanolBleachMurashige And Skoog MediumAgarKanamycinAspiratorVacuum PumpDecantation BottlePolyethylene TubeAquarium Valve

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