JoVE Journal
Biology
Biology
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単核RNAシーケンシングのための成体マウス腎臓からの核分離
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Article
DOI:
10.3791/62901-v
•
06:00 min
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September 20th, 2021
単核RNAシーケンシングのための成体マウス腎臓からの核分離
September 20th, 2021
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Janna Leiz*1,2, Christian Hinze*1,2, Anastasiya Boltengagen3, Caroline Braeuning4, Christine Kocks3, Nikolaus Rajewsky3, Kai M. Schmidt-Ott1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
ここでは、髄質腎細胞タイプの表現を改善し、酵素組織解離による遺伝子発現アーティファクトを回避する凍結マウス腎臓から高品質の核を単離するためのプロトコルを提示します。