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Biology
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単核RNAシーケンシングのための成体マウス腎臓からの核分離
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
単核RNAシーケンシングのための成体マウス腎臓からの核分離
DOI:
10.3791/62901-v
•
06:00 min
•
September 20, 2021
•
Janna Leiz*
1,2
,
Christian Hinze*
1,2
,
Anastasiya Boltengagen
,
Caroline Braeuning
,
Christine Kocks
,
Nikolaus Rajewsky
,
Kai M. Schmidt-Ott
2
1
Department of Nephrology and Medical Intensive Care Medicine
,
Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin
,
2
Molecular and Translational Kidney Research
,
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC)
,
3
Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements
,
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC)
,
4
Berlin Institute of Medical Systems Biology (BIMSB)
,
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Chapters
00:04
Introduction
00:41
Tissue Preparation
01:41
Tissue Homogenization and Cell Lysis
03:11
Nuclei Purification
04:40
Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney
05:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、髄質腎細胞タイプの表現を改善し、酵素組織解離による遺伝子発現アーティファクトを回避する凍結マウス腎臓から高品質の核を単離するためのプロトコルを提示します。
Tags
Nuclei Isolation
Single-nucleus RNA-sequencing
Cortex
Medulla
RNA Stabilization
Nuclei Lysis Buffer
Cell Lysis
Cell Straining
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