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1Department of Nephrology and Medical Intensive Care Medicine,Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Chapters
- 00:04Introduction
- 00:41Tissue Preparation
- 01:41Tissue Homogenization and Cell Lysis
- 03:11Nuclei Purification
- 04:40Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney
- 05:30Conclusion
Ici, nous présentons un protocole pour isoler des noyaux de haute qualité à partir de reins de souris congelés qui améliorent la représentation des types de cellules rénales médullaires et évitent les artefacts d’expression génique de la dissociation des tissus enzymatiques.
