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Live-Cell Imaging af transskriptionel aktivitet på DNA Double-Strand Pauser

Article DOI: 10.3791/62968-v • 09:07 min • September 20th, 2021
September 20th, 2021 •
1, 1, 1, 1
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa

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Denne protokol præsenterer et nyt reporter-gensystem og den eksperimentelle opsætning til at opdage transskription ved DNA-dobbeltstrengsbrud med enkeltmolekylefølsomhed.

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Biologi Udgave 175 Transskription DNA-skader DNA dobbeltstrenget brud RNA enkeltmolekyle billeddannelse promotor exon break-induceret transskription
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