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Chapters
- 00:04Introduction
- 00:53Preparation and Transfection of Cells for Live-Cell Microscopy
- 02:09Experimental Setup
- 04:05Image Acquisition
- 06:04Microscopy Calibration Measurements and Analysis
- 06:51Results: Analysis of Transcription Detection at Sites of DNA Double-Strand Breaks
- 08:12Conclusion
Tags
Keywords: Live-cell ImagingTranscriptional ActivityDNA Double-strand BreaksTranscription DetectionSingle-cell AnalysisDNA Damage ResponseTranscription-replication CrosstalkDoxycycline InductionCalibration MeasurementsMicrocentrifuge Tube PreparationTransfectionGlass Bottom DishMicroscopy Observation
