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多光子活体成像用于监测小鼠后肢模型中动脉生成过程中白细胞募集
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Medicine
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JoVE Journal Medicine
Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
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多光子活体成像用于监测小鼠后肢模型中动脉生成过程中白细胞募集

DOI:
10.3791/62969-v

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07:50 min

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September 30, 2021

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, , , , ,
1Walter-Brendel-Centre of Experimental Medicine, University Hospital,Ludwig-Maximilians-Universität München, 2Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital,Ludwig-Maximilians-Universität München, 3Biomedical Center, Institute of Cardiovascular Physiology and Pathophysiology, Faculty of Medicine,Ludwig-Maximilians-Universität München, 4TKI,University of Bern, 5Department of Internal Medicine I and Cardiology, University Hospital,Ludwig-Maximilians-Universität München

Chapters

  • 00:05Introduction
  • 01:02Tissue Preparation
  • 02:18Intravital Multiphoton Microscopy
  • 06:12Results: Implications of VivoFollow Drift Correction Software
  • 07:05Conclusion

Summary

Automatic Translation

Automatic Translation

白细胞和血小板的募集是动脉生成过程中侧支动脉有效生长所必需的基本组成部分。多光子显微镜是一种有效的工具，用于跟踪体内 高 时空分辨率和较少光毒性的细胞动力学，以研究动脉生成过程中的白细胞募集和外渗。

Tags

Multiphoton Intravital ImagingLeukocyte RecruitmentArteriogenesisMurine Hindlimb ModelCell DynamicsMicroscopySurgical ProcedureCollateral VesselsAdductor MuscleFluorescence ImagingTimelapse Imaging

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