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Multiphoton intravital billeddannelse til overvågning af leukocytrekruttering under arterogenese i en murin bagbensmodel
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Multiphoton intravital billeddannelse til overvågning af leukocytrekruttering under arterogenese i en murin bagbensmodel

Article DOI: 10.3791/62969-v • 07:50 min • September 30th, 2021
September 30th, 2021 •
1,2,3, 4, 1,5, 1,3, 1,3, 1,5
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-Universität München, 2Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital, Ludwig-Maximilians-Universität München, 3Biomedical Center, Institute of Cardiovascular Physiology and Pathophysiology, Faculty of Medicine, Ludwig-Maximilians-Universität München, 4TKI, University of Bern, 5Department of Internal Medicine I and Cardiology, University Hospital, Ludwig-Maximilians-Universität München

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Rekruttering af leukocytter og blodplader udgør en væsentlig komponent, der er nødvendig for effektiv vækst af sikkerhedsarterier under arteriogenese. Multifotonmikroskopi er et effektivt værktøj til sporing af celledynamik med høj rumlig-tidsmæssig opløsning in vivo og mindre fototoksicitet til undersøgelse af leukocytrekruttering og ekstravasation under arteriogenese.

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Denne måned i JoVE udgave 175 Multiphoton intravital billeddannelse arteriogenese lårbensarteriel ligering leukocyt levende handel leukocyt ekstravasation drift korrektion værktøjer ViVoFollow drift korrektion software væv bevægelse
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