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Journal
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Neuroscience
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マウス脳海馬組織の機械的解離と酵素的解離の複合
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
Please note that all translations are automatically generated.
Click here for the English version.
マウス脳海馬組織の機械的解離と酵素的解離の複合
DOI:
10.3791/63007-v
•
07:14 min
•
October 21, 2021
•
Madison Trujillo
2,3
,
Taylor McElroy
2,3
,
Taurean Brown
2,3
,
Pilar Simmons
2
,
Fabio Ntagwabira
2,3
,
Antiño R. Allen
2,3
1
Division of Radiation Health
,
University of Arkansas for Medical Sciences
,
2
Department of Pharmaceutical Sciences
,
University of Arkansas for Medical Sciences
,
3
Neurobiology & Developmental Sciences
,
University of Arkansas for Medical Sciences
Chapters
00:04
Introduction
00:50
Perfusion
02:01
Dissection
03:11
Adult Brain Dissociation
04:15
Debris Removal
05:28
Live and Dead Staining
06:00
Results: Flow Cytometry Analysis Following Automated Mechanical Dissociation and Enzymatic Digestion of Mouse Brain Hippocampal Tissue
06:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
この神経細胞解離プロトコルは、出発物質の量が少ないサンプルを対象としており、オプションの固定および染色ステップで、下流分析用の生存率の高い単一細胞懸濁液を生成します。
Tags
Keywords: Neural Dissociation
Single-cell Suspension
Mouse Brain
Hippocampus
Enzymatic Dissociation
Mechanical Dissociation
Perfusion
Brain Extraction
Sample Preparation
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