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通过显微注射在类 圆线虫 物种中产生转基因和敲除
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
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通过显微注射在类 圆线虫 物种中产生转基因和敲除

DOI:
10.3791/63023-v

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09:42 min

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October 07, 2021

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,
1Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles

Chapters

  • 00:05Introduction
  • 00:37Preparation of Microinjection Slides
  • 01:19Collection of Young Adult Strongyloides for Microinjection
  • 03:19Preparing the Microscope and Breaking the Needle
  • 05:27Microinjecting Strongyloides
  • 07:22Collecting and Screening F1 Larvae to Recover Transgenics/Knockouts
  • 08:31Results: Generating Transgenics and Knockouts in Strongyloides Species Using Intragonadal Microinjection
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

寄生线虫 类圆线 虫粪类和大 鼠类圆线 虫的功能基因组工具包包括转基因、CRISPR/Cas9 介导的诱变和 RNAi。该协议将演示如何使用性腺内显微注射将转基因和CRISPR成分引入 粪类猪笼草 和 鼠李糖杆菌中。

Tags

MicroinjectionTransgenicsKnockoutsStrongyloidesParasitesWormsBaermannFecal-charcoalNematode Growth MediumE. Coli HB101

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