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Journal
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Cancer Research
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小儿弥漫性中线胶质瘤的活-3D细胞免疫细胞化学测定
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Cancer Research
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JoVE Journal
Cancer Research
Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma
Please note that all translations are automatically generated.
Click here for the English version.
小儿弥漫性中线胶质瘤的活-3D细胞免疫细胞化学测定
DOI:
10.3791/63091-v
•
09:06 min
•
November 11, 2021
•
Giulia Pericoli*
1
,
Roberta Ferretti*
1
,
Andrew S. Moore
3
,
Maria Vinci
1
Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children’s Hospital
,
IRCCS
,
2
Oncology Service
,
Children’s Health Queensland Hospital and Health Service
,
3
Child Health Research Centre
,
The University of Queensland
Chapters
00:05
Introduction
01:04
ALR/Antibody Complex Preparation and Invasion Assay Setup
03:25
Migration Assay Setup
04:18
Live-Cell Analysis Instrument Setting for Image Acquisition
05:14
Live-Cell Analysis Instrument Setting for Image Analysis
06:30
Results: Analysis of CD44 Expression in 3D Tumor Cell Invasion and Migration
08:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究提出了一种应用于儿科弥漫性中线胶质瘤细胞系的活 3D 细胞免疫细胞化学方案,有助于实时研究 3D 细胞侵袭和迁移等动态过程中质膜上蛋白质的表达。
Tags
Live Cell Imaging
3D Cell Culture
Immunocytochemistry
Diffuse Midline Glioma
CD44
Cell Migration
Cell Invasion
Molecular Target
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