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JoVE Journal Neuroscience
Kvantificering af reaktive iltarter ved anvendelse af 2′,7′-dichlorfluoresceindiacetafet sonde og flowcytometri i Müller glialceller
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Kvantificering af reaktive iltarter ved anvendelse af 2′,7′-dichlorfluoresceindiacetafet sonde og flowcytometri i Müller glialceller

Article DOI: 10.3791/63337-v • 14:25 min • May 13th, 2022
May 13th, 2022 •
1,2, 1,3, 1,2, 1,2, 1,2
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

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Her foreslår vi en systematiseret, tilgængelig og reproducerbar protokol til påvisning af cellulære reaktive iltarter (ROS) ved hjælp af 2′,7′-dichlorfluoresceindiacetersonde (DCFH-DA) i Müller glialceller (MMC'er). Denne metode kvantificerer de samlede cellulære ROS-niveauer med et flowcytometer. Denne protokol er meget nem at bruge, egnet og reproducerbar.

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Neurovidenskab udgave 183
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