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Developmental Biology
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次世代シーケンシングのためのマウス網膜内皮細胞の単離
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JoVE Journal
Developmental Biology
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
次世代シーケンシングのためのマウス網膜内皮細胞の単離
DOI:
10.3791/63133-v
•
09:59 min
•
October 11, 2021
•
Nicholas W. Chavkin
2
,
Shelby Cain
,
Kenneth Walsh
3,4
,
Karen K. Hirschi
2,4,5
1
Department of Cell Biology
,
University of Virginia School of Medicine
,
2
Robert M. Berne Cardiovascular Research Center
,
University of Virginia School of Medicine
,
3
Department of Cardiology
,
University of Virginia School of Medicine
,
4
Hematovascular Biology Center
,
University of Virginia School of Medicine
,
5
Yale Cardiovascular Research Center
,
Yale University School of Medicine
Chapters
00:04
Introduction
00:38
Obtain Mouse Eyes for Retinal Isolation and Isolate Mouse Retinal Tissue
02:30
Digest Retinal Tissue into a Single Cell Suspension and Immunostain the Cells with Antibodies
04:07
Prepare for Fluorescence‐Activated Cell Sorting
05:19
Isolate Viable Endothelial Cells via FACS
07:18
Results: Analysis of Isolated Endothelial Cells and Next‐Generation Sequencing After Isolation
09:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、細胞収量、純度、および生存率に最適化されたマウス生後網膜内皮細胞の単離方法について説明しています。これらの細胞は、次世代シーケンシングアプローチに適しています。
Tags
Retinal Endothelial Cells
Next-generation Sequencing
Vascular Development
Neonatal Mouse
Eye Dissection
Cell Isolation
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